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ЗООТЕХНИЯ И ВЕТЕРИНАРИЯ

Список источников

1.Grechkina B.V., Miroshnikov S.A., Lebedev S.V. Lipid spectrum of blood when vegetable fats are introduced into the diet of calves // IOP Conf. Earth En-viron. Sci. 2021. №624. P. 012025.

2.Sheida E.V., Lebedev S.V., Vershinina I.A., Grechkina V.V., Gubaidullina I.Z. Assessment of the quality and safety of the use of vegetable fats in meat production // IOP Conf. Earth Environ. Sci. 2021. №624. P. 012113.

3.Dolmatova I., Valitov F., Gizatullin R., Khaziev D., Sedykh T., Kharla-mov A. Effect of the bovine tg5 gene polymorphism on milkand meat-producing abil-ity // Vet. World. 2020. №13(10). P. 2046–2052.

4.Седых Т.А., Калашникова Л.А., Гизатуллин Р.С., Косилов В.И. Качество туш мясного скота различных генотипов по гену тиреоглобулина (TG5) // Зоотехния. 2020. №7. С. 4–8.

5.Селионова М.И., Плахтюкова В.Р. Мясная продуктивность бычков казахской белоголовой породы разных геноти- пов по генам CAPN1 и GH // Молочное и мясное скотоводство. 2020. №4. С. 9–12.

6.Raza S.H.A., Shijun L., Khan R., Schreurs N.M., Manzari Z., El-Aziz A.H.A., Ullah I., Kaster N., Shah M.A., Zan L. Polymorphism of the PLIN1 gene and its association with body measures and ultrasound carcass traits in Qinchuan beef cattle // Genome. 2020. №63(10). P. 483–492.

7.Giblin L., Stephen T.B., Kearney B.M., Waters S.M., Callanan M.J., Ber-ry D.P. Association of bovine leptin polymorphisms with energy output and energy stor-age traits in progeny tested Holstein-Friesian dairy cattle sires // BMC Genet. 2010. №11(1). P. 73.

8.Komisarek J. Impact of LEP and LEPR gene polymorphisms on func-tional traits in Polish Holstein-Friesian cattle // Anim. Sci. Pap. Rep. 2010. №28(2). P. 133–141.

9.Sedykh T.A., Kalashnikova L.A., Gusev I.V., Pavlova I.Y., Gizatullin R.S., Dolmatova I.Y. Influence of TG5 and LEP gene polymorphism on quantitative and qualitative meat composition in beef calves // Iraqi J. Vet. Sci. 2016. №30(2). P. 41–48.

10.Liefers S.C., te Pas M.F.W., Veerkamp R.F., Chilliard Y., Delavaud C., Gerritsen R., van der Lende T. Association of leptin gene polymorphisms with serum leptin concentration in dairy cows // Mamm. Genome. 2003. №14(9). P. 657–663.

11.Nkrumah J.D., Li C., Yu J., Hansen C., Keisler D.H., Moore S.S. Poly-morphism in the bovine leptin gene promoter associated with serum leptin concentration, growth, feed intake, feeding behavior, and measures of carcass merit // J. Anim. Sci. 2005. №83(1). P. 20–28.

12.Williams G.L., Amstalden M., Garcia M.R., Stanko R.L., Nizielski S.E., Morrison C.D., Keisler D.H. Leptin and its role in the central regulation of reproduction in cattle // Domest. Anim. Endocrinol. 2002. №23(1–2). P. 339–349.

13.Geary T.W., McFadin E.L., MacNeil M.D., Grings E.E., Short R.E., Funston R.N., Keisler D.H. Leptin as a predictor of carcass composition in beef cattle // J. Anim. Sci. 2003. №81(1). P. 1–8.

14.Nkrumah J.D., Keisler D.H., Crews D.H. Jr., Basarab J.A., Wang Z., Li C., Price M.A., Okine E.K., Moore S.S. Genetic and phenotypic relationships of serum leptin concentration with performance, efficiency of gain, and carcass merit of feedlot cattle // J. Anim. Sci. 2007. №85(9). P. 2147–2155.

15.Buchanan F.C., Fitzsimmons C.J., Van Kessel A.G., Thue T.D., Win-Kelman-Sim D.C., Schmutz S.M. Association of a missense mutation in the bovine lep-tin gene with carcass fat content and leptin mRNA levels // Genet. Sel. Evol. 2002. №34(1). P. 105–116.

GENETIC AND PHYSIOLOGICAL FACTORS OF ADIPOSE TISSUE FORMATION IN ABERDEEN-ANGUS HEIFERS AND FIRST-CALF COWS

©2022. Nikolai P. Gerasimov1, Kinispay M. Dzhulamanov2, Vladimir I. Kolpakov3,

1,2,3 Federal Research Center for Biological Systems and Agrotechnologies of the Russian Academy of Sciences, Orenburg, Russia

1nick.gerasimov@rambler.ru

Abstract. Fat formation in beef mature herds is an inefficient process from an energy and economic point of view, since a lot of feed energy is spent on the synthesis of adipose tissue. The aim of the research was to study the effect of polymorphisms in leptin gene and pregnancy on the adipose tissue formation in Aberdeen-Angus heifers and first-calf cows. Heifers (n=49) and first-calf cows (n=30) were grouped according to their genotype for LEP C528T and LEP C73T polymorphisms at the age of 24 months. Heterozygous heifers with the LEP528CT genotype had a superiority in weight and yield of visceral fat by 1.22-2.48 kg and 0.15-0.18%. The maximum weight and yield of internal fat was found in heifers with the homozygous LEP 73CC genotype. Heterozygous heifers for the studied polymorphisms of leptin gene had the leadership in terms of subcutaneous adipose tissue formation. Chemical analysis of slaughter products showed the superiority of LEP 528CT and LEP 73CT individuals in fat synthesis. The intramuscular fat content in longissimus dorsi muscle was 0.09-0.15% and 0.25-0.26%, and in the average sample of ground beef it was 1.47-1.69% and 0.10- 0.68% more compared with homozygous peers, respectively. Pregnancy had a more significant effect on slaughter parameters and

ЗООТЕХНИЯ И ВЕТЕРИНАРИЯ

adipose tissue formation than studied polymorphisms. First-calf cows had a significantly lower fat content in carcasses than heifers.

Keywords: Aberdeen-Angus breed, heifers, first-calf cows, pregnancy, genotype, leptin gene, polymorphism, adipose tissue

References

1.Grechkina B.V., Miroshnikov S.A., Lebedev S.V. Lipid spectrum of blood when vegetable fats are introduced into the diet of calves, IOP Conf. Earth Environ. Sci., 2021, No. 624. P. 012025.

2.Sheida E.V., Lebedev S.V., Vershinina I.A., Grechkina V.V., Gubaidullina I.Z. Assessment of the quality and safety of the use of vegetable fats in meat production, IOP Conf. Earth Environ. Sci., 2021, No. 624, P. 012113.

3.Dolmatova I., Valitov F., Gizatullin R., Khaziev D., Sedykh T., Kharlamov A. Effect of the bovine tg5 gene polymorphism on milkand meat-producing ability, Vet. World, 2020, No. 13(10), P. 2046–2052.

4.Sedykh T.A., Kalashnikova L.A., Gizatullin R.S., Kosilov V.I. The quality of carcasses of beef cattle of different genotypes of the thyroglobulin (TG5) gene, Zootechniya, 2020, No. 7, P. 4–8.

5.Selionova M.I., Plakhtyukova V.R. Meat productivity of Kazakh Whiteheaded steers of different genotypes by genes CAPN1 and GH, Dairy and Beef Cattle Breed, 2020, No. 4, P. 9–12.

6.Raza S.H.A., Shijun L., Khan R., Schreurs N.M., Manzari Z., El-Aziz A.H.A., Ullah I., Kaster N., Shah M.A., Zan L. Polymorphism of the PLIN1 gene and its association with body measures and ultrasound carcass traits in Qinchuan beef cattle, Genome, 2020, No. 63(10), P. 483–492.

7.Giblin L., Stephen T.B., Kearney B.M., Waters S.M., Callanan M.J., Berry D.P. Association of bovine leptin polymorphisms with energy output and energy storage traits in progeny tested Holstein-Friesian dairy cattle sires, BMC Genet., 2010, No. 11(1), P. 73.

8.Komisarek J. Impact of LEP and LEPR gene polymorphisms on functional traits in Polish Holstein-Friesian cattle, Anim. Sci. Pap. Rep., 2010, No. 28(2), P. 133–141.

9.Sedykh T.A., Kalashnikova L.A., Gusev I.V., Pavlova I.Y., Gizatullin R.S., Dolmatova I.Y. Influence of TG5 and LEP gene polymorphism on quantitative and qualitative meat composition in beef calves, Iraqi J. Vet. Sci., 2016, No. 30(2), P. 41–48.

10.Liefers S.C., te Pas M.F.W., Veerkamp R.F., Chilliard Y., Delavaud C., Gerritsen R., van der Lende T. Association of leptin gene polymorphisms with serum leptin concentration in dairy cows, Mamm. Genome, 2003, No. 14(9), P. 657–663.

11.Nkrumah J.D., Li C., Yu J., Hansen C., Keisler D.H., Moore S.S. Polymorphism in the bovine leptin gene promoter associated with serum leptin concentration, growth, feed intake, feeding behavior, and measures of carcass merit, J. Anim. Sci., 2005, No. 83(1), P. 20–28.

12.Williams G.L., Amstalden M., Garcia M.R., Stanko R.L., Nizielski S.E., Morrison C.D., Keisler D.H. Leptin and its role in the central regulation of reproduction in cattle, Domest. Anim. Endocrinol., 2002, No. 23(1–2), P. 339–349.

13.Geary T.W., McFadin E.L., MacNeil M.D., Grings E.E., Short R.E., Funston R.N., Keisler D.H. Leptin as a predictor of carcass composition in beef cattle, J. Anim. Sci., 2003, No. 81(1), P. 1–8.

14.Nkrumah J.D., Keisler D.H., Crews D.H. Jr., Basarab J.A., Wang Z., Li C., Price M.A., Okine E.K., Moore S.S. Genetic and phenotypic relationships of serum leptin concentration with performance, efficiency of gain, and carcass merit of feedlot cattle, J. Anim. Sci., 2007, No. 85(9), P. 2147–2155.

15.Buchanan F.C., Fitzsimmons C.J., Van Kessel A.G., Thue T.D., Win-Kelman-Sim D.C., Schmutz S.M. Association of a missense mutation in the bovine leptin gene with carcass fat content and leptin mRNA levels, Genet. Sel. Evol., 2002, No. 34(1), P. 105–116.

Сведения об авторах

Н. П. Герасимов 1 – д-р биол. наук, старший научный сотрудник; К. М. Джуламанов 2 – д-р с.-х. наук, руководитель селекционно-генетического центра по мясным породам скота; В. И. Колпаков3 – канд. с.-х. наук, научный сотрудник.

1,2Федеральный научный центр биологических систем и агротехнологий Российской Академии наук, ул. 9 января 29, г. Оренбург, Россия.

1 nick.gerasimov@rambler.ru

2 kinispai.d@yandex.ru

3 vkolpakov056@yandex.ru

Information about the authors

N. P. Gerasimov 1 – Dr. Biol. Sci., Senior Rresearcher; K. M. Dzhulamanov 2 – Dr. Agr. Sci., Head of department; V. I. Kolpakov – Cand. Agr. Sci., Researcher.

1,2Federal Research Centre of Biological Systems and Agrotechnologies of the Russian Academy of Sciences, 29, 9 Yanvarya St., Orenburg, Russia.

1 nick.gerasimov@rambler.ru

2 kinispai.d@yandex.ru

3 vkolpakov056@yandex.ru

Конфликт интересов: авторы заявляют об отсутствии конфликта интересов.

Conflict of interest: the authors declare that they have no conflicts of interest.

Статья поступила в редакцию 25.07.2022; одобрена после рецензирования 12.10.2022; принята к публикации 25.11.2022. The article was submitted 25.07.2022; approved after reviewing 12.10.2022; accepted for publication 25.11.2022.

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Пермский аграрный вестник №4 (40) 2022 Perm Agrarian Journal. 2022; 4 (40)

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Быки

Коровы

Потомство

Расстояние объед

Рис 1. Рисунок. Дендрограмма взаимоотношений между родителями и потомками по аллелям генов GHR, IGF-I и GDF5

Fig. 1. Drawing. Dendrogram of relationships between parents and descendants from alleles of genes GHR, IGF-I and GDF5

Ассоциации полиморфизма генов GHR, IGF-I и GDF5 с линейными промерами (см) и индексами телосложения (%) потомков (телок) герефордской породы

Perm Agrarian Journal. 2022; 4 (40)

ЗООТЕХНИЯ И ВЕТЕРИНАРИЯ

INDICATORS OF PARENTAL BREEDING VALUE

WITH CONSIDERATION OF THE POLYMORPHISM OF GHR, IGF-I AND GDF5 GENES IN HEREFORD CATTLE

©2022. Marina P. Dubovskova,

Federal Research Center for Biological Systems and Agrotechnologies of the Russian Academy of Sciences, Orenburg, Russia, dubovskova.m@mail.ru

Abstract. The study of allelic profiles in gene markers of productivity with considering breeding traits in dynamics from parents to offspring will allow selection in generations at an early age. The aim of the work was to study the genetic structure of the herd and the breeding value of the parents, taking into account the effect of polymorphisms in GHR, IGF-I and GDF5 genes on the body size of the offspring (heifers). Analysis of polymorphism showed that the genetic frequencies in the population varied from 0.045±0.044 (GDF5TT in offspring) to 0.875±0.117 (GDF5CC in cows and sires). Young animals inherited the alleles of the studied genes. The frequency of the desired allele B of the IGF-I gene in offspring was 3.9% higher compared to sires, but less by 8.6% in dams. The frequencies of GDF5TT and GHRFF homozygotes in young animals was less by 8.0-20.5% compared with parents. It was found that the offspring and dams were genetically closest, the distance was low (0.114 units), sires were more distant (0.125 units). Carriers of heterozygotes (heifers of I group) were superior in withers and hip height, chest girth, oblique body length and half-girth of the back by 3.0-5.7 cm (2.3-4.1%) compared with peers of homozygous genotypes (group II). The tendency of the advantage of heifers from group I was expressed in terms of massiveness and compactness indices (by 1.7% and 1.9%), but they were at the same level in terms of meatiness index, and they were inferior to their peers by 0.6% in terms of lengthiness. Inheritance by offspring of different polymorphic states of marker genes of meat productivity was indicated in differences in the external parameters of animals.

Keywords: Hereford breed, genotype, polymorphism, GHR, IGF-I and GDF5 genes, linear measurements, body conformation indices

References

1.M.I. Selionova, L.N. Chizhova, G.T. Bobryshov et al. Perspective genetic markers of horned cattle, Agricultural Bulletin of Stavropol Region, 2018, No. 3 (31). P. 44-51. DOI: 10.31279/2222-9345-2018-7-31-44-51

2.Novikov A.A., Semak M.S., Kalashnikova L.A. The need to improve the system of genetic expertise of breeding products in the Russian Federation, Zootechniya, 2021, No. 6. P. 2-6. DOI: 10.25708/ZT.2021.17.85.001

3.Tyulebaev SD, Kadysheva MD, Kosilov VI, Gabidulin VM. The state of polymorphism of genes affecting the meat quality in micropopulations of meat Simmentals, IOP Conference Series: Earth and Environmental Science, 2021, No. 624. P. 012045. doi:10.1088/1755-1315/624/1/012045.

4.Dzhulamanov K.M., Gerasimov N.P., Kolpakov V.I., Dzhulamanov E.B. Age variability of the constitution and exterior of the Hereford bulls genotyped for the gdf5 gene, Animal husbandry and fodder production, 2020, Vol. 103. No. 3. P. 36-

45.DOI: 10.33284/2658-3135-103-3-36

5.Liu Y., Jiao Y., Zan L., Xin Y., Li L., Tian W. Molecular characterization, polymorphism of growth differentiation factor 5 gene and association with ultrasound measurement traits in native Chinese cattle breeds, African Journal of Biotechnology, 2010, Vol. 9(33). pp. 5269-5273.

6.Ardicli S, Samli H, Dincel D, Soyudal B, Balci F. Individual and combined effects of CAPN1, CAST, LEP and GHR gene polymorphisms on carcass characteristics and meat quality in Holstein bulls, Arch. Anim. Breed., 2017, Vol. 60(3). P. 303-313. DOI: 10.5194/aab-60-303-2017

7.Dzhulamanov K.M., Selionova M.I., Gerasimov N.P. The genetic characteristic of Hereford cattle population, Vestnik Bashkir State Agrarian University, 2018, No. 4 (48). P. 59-64.

8.Dzhulamanov K.M., Gerasimov N.P. The formation of meat productivity of Hereford bulls of different body types in conjunction with environmental factors, Animal husbandry and fodder production, 2020, Vol. 103. No.2. P. 57-67. DOI: 10.33284/2658-3135-103-2-57

9.Beishova I.S. The polymorphism of genes of the somatotrophic cascade associated with meat productivity in Kazakh White-headed cows, Bulletin Samara State Agricultural Academy, 2018, No. 69(1). Pp. 58-62.

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ЗООТЕХНИЯ И ВЕТЕРИНАРИЯ

10. Gabidulin V.M., Alimova S.A. Selection and genetic parameters of economically useful traits genotyped young Aber- deen-Angus cattle by genes CАPN1 CAST and bGH, Izvestiya of Orenburg State Agrarian University, 2021, No. 6(92). P. 289-294.

Сведения об авторах

М.П. Дубовскова – д-р с.-х. наук, старший научный сотрудник.

Федеральный научный центр биологических систем и агротехнологий Российской академии наук, ул. 9 Января, 29, г. Оренбург, Россия, 460000

Information about the authors

M.P. Dubovskova – Dr. Agr. Sci., Senior Researcher;

Federal Scientific Center for Biological Systems and Agrotechnologies of the Russian Academy of Sciences, 29, 9 Yanvarya st., Orenburg, Russia, 460000

Конфликт интересов: авторы заявляют об отсутствии конфликта интересов.

Conflict of interest: the authors declare that they have no conflicts of interest.

Статья поступила в редакцию 09.09.2022; одобрена после рецензирования 12.10.2022; принята к публикации 25.11.2022 The article was submitted 09.09.2022; approved after reviewing 12.10.2022; accepted for publication 25.11.2022