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4.Lucy MC Reproductive loss in high-producing dairy cattle: where will it end? Journal of Dairy Science 84 1277-1293 (2011)

5.Mann GE, Fray MD, Robinson RS, Wathes DC and Lamming GE Interferon tau production during early pregnancy in the cow Biology of Reproduction 66 Supplement {Abstract 120) (2012)

6.McArdle CA and Holtorf AP Oxytocin and progesterone release from bovine corpus luteal cells in culture: effects of insulinlike growth factor |, insulin and prostaglandins Endocrinology 124 1278-1286 (2009)

7.Nanda AS, Ward WR and Dobson H (2009) The relationship between milk yield and cystic ovarian disease in cattle British Veterinary journal 145 39-45 (2012)

8.O'Callaghan D, Lozano JM, Fahey}, Gath V, Snijders S and Boland MP Relationships between nutrition and fertility in dairy cattle British Society of Animai Science Occasional Publication 26 147-160 (2011)

9.Opsomer G, Coryn M, Deluyker H and de Kruif A (2008) An analays is of ovarian dysfunction in high yielding dairy cows after calving based on progesterone profiles Reproduction in Domestic Animals 33 193-204 (2008)

10.Pushpakumara PGA, Robinson RS, Demmers KJ, Mann GE, Sinclair KD, Webb R and Wathes DC Expression of the insulinlike growth factor (IGF) system in the bovine oviduct at oestrus and during early pregnancy Reproduction 123 859-868 (2012)

11.Roche F and Diskin MG Resumption of reproductive activity in the early postpartum period of cows British Society of Animal Science Occasional Publication 26 31-42 (2011)

12.Snyder JL, Clapper JA, Roberts AJ, Sanson DW, Hamernik DL and Moss GE Insulin-like growth factor-l, insulin-like growth factor binding Ovarian function and embryo survival in postpartum cow’s proteins, and gonadotrophins in the hypothalamic— pituitary axis and serum of nutrient restricted ewes Biology of Reproduction 61 219-224 (2009)

13.Starbuck GR, Darwash AO, Mann GE and Lamming GE The detection and treatment of post insemination progesterone insufficiency in dairy cows British Society of Animal Science Occasional Publication 26 447-450 237 (2011)

14.Starbuck GR, Gutierrez CG and Mann GE Relationships between preand post-ovulatory steroidogenic function in vivo in cattle Journal of Reproduction and Fertility Abstract Series 25 (Abstract 55) (2010)

15.Wathes DC, Beever DE, Cheng Z, Pushpakumara PGA and Taylor VJ Lifetime organization and management of reproduction in the dairy cow British Society of Animal Science Occasional Publication 28 59-69 (2011)

RESYNCHRONIZATION OF COWS AND HEIFERS RECIPIENTS ACCORDING TO THE CIDR-SYNCH PROTOCOL IN COMBINATION WITH

INJECTION OF MESENCHYMAL STEM CELLS (MSC)

©2022. Victor Y. Tkachev1 , Tatyana V. Zubova 2, Vladimir A. Pleshkov 3

1 Vaganovo Agricultural Research Center, Kuzbass, Russia

1,2,3 Kuzbass State Agricultural Academy, Kemerovo, Russia,

2suta54@mail.ru

Abstract. The purpose of this study was to increase the percentage of pregnancy of Holstein cows and heifers by timely in vitro embryo transfer to recipients. The animals were prepared using the CIDR-SYNCH protocol (progesterone spiral), supplemented by injection of mesenchymal stem cells included in the preparation Bovi Stem (protein-peptide complex obtained from conditioned medium during cultivation of mesenchymal stem cells of cattle). We considered factors affecting the interval to ovulation and the adequacy of luteal function, special attention was paid to potential metabolic signals that can affect the activity of the ovaries. The scientific and economic experiment was conducted in the spring of 2022 on the basis of JSC Vaganovo (Kemerovskaya Oblast, Promyslennovsky District). Animals of the experimental group were given a 3-fold injection with Bovi Stem, followed by CIDR-SYNCH synchronization. Then, on the 7th day of the cycle, after assessing the structure and volume of the corpus luteum, embryos were transplanted in vitro at the early blastocyst stage. On the 1st and 4th day of the experiment, the level of somatotropin and insulin in the blood was assessed. On the 28th day of the experiment, according to the results of ultrasound diagnostics, pregnancy was diagnosed in cows and heifers. During the study, it was found that the strategy of resynchronization of cows and heifers of Holstein breed recipients according to the CIDR-SYNCH protocol in combination with a three-fold series of injections of mesenchymal stem cells (MSC), allows the

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increase in the concentration of progesterone levels in the blood of recipients, by improving metabolic processes in the body affecting the quality of folliculogenesis, which has a beneficial effect on the quality of implantation after embryo transplantation.

Keywords: mesenchymal stem cells, progesterone, somatotropin, recipient, embryos, transplantation, resynchronization

Referenсes

1.Adam CL, Gadd TS, Findlay PA and Wathes DC Insulin-like growth factor (IGF)-] stimulation of luteinizing hormone secretion, and expression of mRNAs for IGFs, their receptors and binding proteins in the ovine pituitary gland Journal of Endocrinology 166 247-254(2010)

2.Butler WR Nutritional effects on resumption of ovarian cyclicity and conception rate in postpartum dairy cows British Society of Animal Science Occasional Publication 26 133-146 (2011)

3.Inskeep EK, Braden TD, Lewis PE and Niswender GD Receptors for luteinizing hormone and follicle stimulating hormone in largest follicles of postpartum beef cows Biology of Reproduction 38 587-591 (2008)

4.Lucy MC Reproductive loss in high-producing dairy cattle: where will it end? Journal of Dairy Science 84 1277-1293

(2011)

5.Mann GE, Fray MD, Robinson RS, Wathes DC and Lamming GE Interferon tau production during early pregnancy in the cow Biology of Reproduction 66 Supplement {Abstract 120) (2012)

6.McArdle CA and Holtorf AP Oxytocin and progesterone release from bovine corpus luteal cells in culture: effects of insulinlike growth factor |, insulin and prostaglandins Endocrinology 124 1278-1286 (2009)

7.Nanda AS, Ward WR and Dobson H (2009) The relationship between milk yield and cystic ovarian disease in cattle British Veterinary journal 145 39-45 (2012)

8.O'Callaghan D, Lozano JM, Fahey}, Gath V, Snijders S and Boland MP Relationships between nutrition and fertility in dairy cattle British Society of Animai Science Occasional Publication 26 147-160 (2011)

9.Opsomer G, Coryn M, Deluyker H and de Kruif A (2008) An analays is of ovarian dysfunction in high yielding dairy cows after calving based on progesterone profiles Reproduction in Domestic Animals 33 193-204 (2008)

10.Pushpakumara PGA, Robinson RS, Demmers KJ, Mann GE, Sinclair KD, Webb R and Wathes DC Expression of the insulin-like growth factor (IGF) system in the bovine oviduct at oestrus and during early pregnancy Reproduction 123 859-868 (2012)

11.Roche F and Diskin MG Resumption of reproductive activity in the early postpartum period of cows British Society of Animal Science Occasional Publication 26 31-42 (2011)

12.Snyder JL, Clapper JA, Roberts AJ, Sanson DW, Hamernik DL and Moss GE Insulin-like growth factor-l, insulin-like growth factor binding Ovarian function and embryo survival in postpartum cow’s proteins, and gonadotrophins in the hypothalamic— pituitary axis and serum of nutrient restricted ewes Biology of Reproduction 61 219-224 (2009)

13.Starbuck GR, Darwash AO, Mann GE and Lamming GE The detection and treatment of post insemination progesterone insufficiency in dairy cows British Society of Animal Science Occasional Publication 26 447-450 237 (2011)

14.Starbuck GR, Gutierrez CG and Mann GE Relationships between preand postovulatory steroidogenic function in vivo in cattle Journal of Reproduction and Fertility Abstract Series 25 (Abstract 55) (2010)

15.Wathes DC, Beever DE, Cheng Z, Pushpakumara PGA and Taylor VJ Lifetime organization and management of reproduction in the dairy cow British Society of Animal Science Occasional Publication 28 59-69 (2011)

Сведения об авторах

В.Ю. Ткачев 1 – аспирант;

Т.В. Зубова 2 – д-р биол. наук, доцент; В.А. Плешков3 – канд. с.-х. наук, доцент.

1,2,3 Кузбасская государственная сельскохозяйственная академия, ул. Марковцева, 5, г. Кемерово, Россия,

1 donwitj@yandex.ru

2 suta54@mail.ru

36110699@mail.ru

Information about the authors

V.Y. Tkachev 1 – Postgraduate Student;

T.V. Zubova 2 – Dr. Biol. Sci., Associate Professor;

V.A. Pleshkov 3 – Cand. Agr. Sci., Associate Professor.

1,2,3 Kuzbass State Agricultural Academy, Kemerovo, Russia,

1 donwitj@yandex.ru

2 suta54@mail.ru

36110699@mail.ru

Конфликт интересов: авторы заявляют об отсутствии конфликта интересов.

Conflict of interest: the authors declare that they have no conflicts of interest.

Статья поступила в редакцию 04.07.2022; одобрена после рецензирования 16.09.2022; принята к публикации 25.11.2022. The article was submitted 04.07.2022; approved after reviewing 16.09.2022; accepted for publication 25.11.2022.

ЗООТЕХНИЯ И ВЕТЕРИНАРИЯ

6.Surai P.F., Kochish I.I., Shapovalov S.O. (2018). Superoxide dismutase activity in chicken gut. Program and Summaries. WPSA UK Branch Annual Meeting, Dublin, 2018, p. 010.

7. Surai P.F., Fisinin V.I. Vitagenes in poultry production. Part 1. Technological and environmental stresses // World’s Poultry Science Journal. – 2016a. – № 72. – Р. 721-733.

8.Сурай П.Ф., Кочиш И.И., Фисинин В.И., Грозина А.А., Шацких Е.В. Молекулярные механизмы поддержания здоовья кишечника птицы: Роль микробиоты. Монография. М., Изд-во «Сельскохозяйственные технологии», 2018. 344 с.

9.Bottje, W.G. Oxidative stress and efficiency: The tightrope act of mitochondria in health and disease // J Anim Sci. 2019, pii: skz219.

10.Fisinin V.I., et al. Feeding poultry. Sergiev Posad, 2000. 375 pp.

11.Руководство по кормлению сельскохозяйственной птицы / И.А. Егоров, В.А. Манукян, Т.М. Околелова, Т.Н. Ленкова, Е.А. Андрианова [и др.]. Сергиев Посад: ВНИТИП, 2019. 226 с.

12.Горячева И.Ю., Русанова Т.Ю., Бурмистрова Н.А., Де Саегер С. Иммунохимические методы определения микотоксинов // Журнал аналитической химии. 2009. Т. 64. № 8. С. 788-806.

EXPERIENCE OF USING THE THERMALLY TREATED SHUNGITE IN THE DIETS OF LAYING HENS

©2022. Vladimir I. Trukhachev1, Maria A. Buryakova2, Oksana E. Komarova3, Marina V. Selina4

1,2,3 Russian State Agrarian University - Moscow Timiryazev Agricultural Academy, Moscow, Russia,

4 Moscow State Academy of Veterinary Medicine and Biotechnology - MVA named after K.I. Skryabin, Moscow, Russia

Abstract. Mycotoxicoses are the most relevant and frequently encountered in modern poultry farming. Against the background of chronic intoxication of the poultry organism with mycotoxins, productivity and safety decrease, suppression of immunity, therefore, the farm incurs economic losses. In this regard, the aim of the study was to evaluate the effect of the natural adaptogen "Blackfeed Plus" in the diets of laying hens of the Brown Nick industrial cross. As a result of the conducted studies, the positive effect of the feed additive "Blackfeed Plus" (in a dosage of 1.0 kg / t of compound feed) on the productivity of laying hens of the Brown-Nick cross was established. According to the test results, it is possible to recommend the use of the mineral supplement "Blackfeed Plus" in the conditions of egg poultry farms, especially for use in the diets of laying hens of the 3rd phase of egg laying. During the research, a high potential of true sorption of shungite to mycotoxins, including non-polar ones, was revealed.

Keywords: shungite, mycotoxins, feed stress, laying hens, Brown Nick cross, prevention of mycotoxicosis.

References

1.Bogolyubova N.V., Nekrasov R.V., Zelenchenkova A.A. Antioksidantnyi status i kachestvo myasa u sel'skokhozyaistvennoi ptitsy i zhivotnykh pri stresse i ego korrektsiya s pomoshch'yu adaptogenov razlichnoi prirody (Obzor) (Antioxidant status and quality of meat in poultry and animals under stress and its correction with the help of adaptogens of various nature (Review)), Sel'skokhozyaistvennaya biologiya, 2022, T. 57, No. 4, pp. 628-663.

2.Proudfoot K., Habing G. Social stress as a cause of diseases in farm animals: Current knowledge and future directions, The Veterinary Journal, 2015, V. 206 (1), pp. 15-21.

3.Scanes, C.G. Biology of stress in poultry with emphasis on glucocorticoids and the heterophil to lymphocyte ratio, Poult Sci., 2019, №95(9), pp.. 2208-2215.

4.Surai P.F., Kochish I.I., Fisinin V.I., Juniper D.T. (2019a). Revisiting Oxidative Stress and the Use of Organic Selenium in Dairy Cow Nutrition. Animals (Basel) 9, 7, pii: E462.

5.Surai P.F., Kochish I.I., Fisinin V.I., Kidd M.K. (2019). Antioxidant Defence Systems and Oxidative Stress in Poultry Biology: An Update. Antioxidants (Basel) 8, 7. pii: E235.

6.Surai P.F., Kochish I.I., Shapovalov S.O. (2018). Superoxide dismutase activity in chicken gut. Program and Summaries. WPSA UK Branch Annual Meeting, Dublin, 2018, p. 010.

7.Surai, P.F. Vitagenes in poultry production. Part 1. Technological and environmental stresses, P.F. Surai, V.I. Fisinin, World’s Poultry Science Journal, 2016, No. 72, pp.721-733.

8.Surai P.F., Kochish I.I., Fisinin V.I., Grozina A.A., Shatskikh E.V. Molekulyarnye mekhanizmy podderzhaniya zdorov'ya kishechnika ptitsy: Rol' mikrobioty (Molecular mechanisms of maintaining the health of poultry intestines: The role of microbiota), Monografiya, M., Izd-vo «Sel'skokhozyaistvennye tekhnologii», 2018, 344 р.

ЗООТЕХНИЯ И ВЕТЕРИНАРИЯ

9.Bottje, W.G. Oxidative stress and efficiency: The tightrope act of mitochondria in health and disease, J. Anim. Sci., 2019, pii: skz219.

10.Fisinin V.I., et al. Feeding poultry. Sergiev Posad, 2000, 375 pp.

11.Rukovodstvo po kormleniyu sel'skokhozyaistvennoi ptitsy (Guidelines for feeding poultry), I.A. Egorov, V.A. Manukyan, T.M. Okolelova, T.N. Lenkova, E.A. Andrianova [i dr.], Sergiev Posad, VNITIP, 2019, 226 р.

12.Goryacheva I.Yu., Rusanova T.Yu., Burmistrova N.A., De Saeger S. Immunokhimicheskie metody opredeleniya mikotoksinov (Immunochemical methods for the determination of mycotoxins), Zhurnal analiticheskoi khimii, 2009, T. 64, No. 8, рр. 788-806.

Сведения об авторах

В.И.Трухачев1 – д-р с.-х. наук, профессор, академик РАН; М.А. Бурякова2– канд. с.-х. наук, доцент;

О.Е. Комарова3– аспирант;

М.В. Селина4– канд. пед. наук, доцент.

1,2,3Российский государственный аграрный университет – МСХА имени К.А.Тимирязева, Москва, Россия, 4Московская государственная академия ветеринарной медицины и биотехнологии имени им. К.И. Скрябина, Москва, Росиия

1rector@rgau-msha.ru

2m.buryakova@rgau-msha.ru

3o.komarova@rgau-msha.ru

4selina.marina@gmail.com

Information about the authors

V. I. Trukhachev1– Dr. Agr. Sci., Professor, RAS Academician M.A. Buryakova2– Cand. Agr. Sci., Associate Professor;

O.E. Komarova3– Postgraduate Student;

M.V. Selina4– Cand. Ped. Sci., Associate Professor;

1,2,3 Russian State Agrarian University - Moscow Timiryazev Agricultural Academy, Moscow, Russia,

4 Moscow State Academy of Veterinary Medicine and Biotechnology - MVA named after K.I. Skryabin, Moscow, Russia 1rector@rgau-msha.ru

2m.buryakova@rgau-msha.ru

3o.komarova@rgau-msha.ru

4selina.marina@gmail.com

Конфликт интересов: авторы заявляют об отсутствии конфликта интересов.

Conflict of interest: the authors declare that they have no conflicts of interest.

Статья поступила в редакцию 5.10.2022; одобрена после рецензирования 8.12.2022; принята к публикации 9.12.2022. The article was submitted 5.10.2022; approved after reviewing 8.12.2022; accepted for publication 9.12.2022.

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Научная статья УДК 595.763/.768:577.112.37:613.26,28

doi: 10.47737/2307-2873_2022_40_138

СРАВНИТЕЛЬНАЯ ХАРАКТЕРИСТИКА БЕЛКА ЛИЧИНОК ZOPHOBAS MORIO И МЯСА ЖИВОТНЫХ И ПТИЦ

©2022. Елена Олеговна Чугунова,

Пермский государственный аграрно-технологический университет имени академика Д.Н. Пря-

нишникова, Пермь, Россия, chugunova.elen@yandex.ru

Аннотация. В статье рассмотрена проблема поиска альтернативного источника протеина для питания человека. Целью работы было изучение аминокислотного состава личинок Zophobas morio и их оценка с точки зрения полноценности белка. Материалом для исследования служили личинки Zophobas morio (n=100), в работе использовали титриметрический метод определения общего белка по Къельдалю согласно ГОСТ 25011 и хроматографический метод определения аминокислотного состава по М-02-902-142-07. В результате установили, что массовая доля об- щего белка в испытуемом образце Zophobas morio была равна 19,0±0,06 %, что сопоставимо с количеством белка в говядине и свинине, в различных частях цыплят-бройлеров, в филе индейки

имясе перепелов. В результате изучения аминокислотного профиля личинок ZM установили, что они содержат заменимые аминокислоты (∑=21,043±0,003 г/100 г в расчете на сухое веще- ство), все 8 незаменимых аминокислот (∑=14,257±0,02003 г/100 г в расчете на сухое вещество)

и2 частично заменимые аминокислоты – аргигин и гистидин (∑=4,766±0,01050 г/100 г в расчете на сухое вещество). Глутаминовая кислота количественно превалировала над остальными (5,068±0,0095 г/100 г в расчете на сухое вещество), среди незаменимых аминокислот отмечено максимальное содержание метионина (2,662±0,0060 г/100 г в расчете на сухое вещество) и ми- нимальное – триптофана (0,562±0,0085 г/100 г в расчете на сухое вещество). Лимитирующей аминокислотой в личинках ZM является лизин, остальные аминокислоты имеют скор более 100 %. По аминокислотному профилю ZM проигрывают говядине, а по содержанию отдельных ами- нокислот идентичны свинине и мясу птиц. По коэффициенту распределения аминокислот и био- логической ценности личинки ZM значительно уступают мясу убойных животных и птиц, а по белково-качественному показателю – свинине и мясу птиц. Таким образом, ZM могут служить прекрасным сырьем для получения как незаменимых, особенно метионина и треонина, так и за- менимых аминокислот.

Ключевые слова: личинки Zophobas morio, массовая доля белка, аминокислоты